11/9/2023 0 Comments P element transposon![]() Only certain strains of fruit flies carry P-elements and the severity of gonadal dysgenesis in their daughters varies. For example, a mobile section of DNA known as the P-element causes a condition called gonadal dysgenesis in female fruit flies, leading to infertility. When these “mobile genetic elements” move they may disrupt other genes and cause disease. However, some sections of DNA are able to move from one place in the genome to another. ![]() eLife digestĭNA provides the instructions needed for life, a role that relies on it being a very stable and organized molecule. The Drosophila short Har-P’s and full-length P-elements relationship parallels the MITEs/DNA-transposase in plants and SINEs/LINEs in mammals. Finally, we uncovered P-element-piRNA-directed repression on Har-P’s transmitted paternally to suppress somatic transposition. Although HISR-D lines still contain full-length P-elements, HISR-N lines lost functional P-transposase but retained Har-P’s that when crossed back to P-transposase restores GD induction. In these lines, we discovered a highly truncated P-element variant we named ‘ Har-P’ as the most frequent de novo insertion. To address the mystery of why Har induces severe GD, we bred hybrid Drosophila with Har genomic fragments into the ISO1 background to create HISR-D or HISR-N lines that still cause Dysgenesis or are Non-dysgenic, respectively. Harwich ( Har) strains with P-elements cause severe GD in F1 daughters when Har fathers mate with mothers lacking P-element-piRNAs (i.e. ![]() Without transposon-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in Drosophila called gonadal dysgenesis (GD). ![]()
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